Lab Resources

SOC "Super" Optimized with Catabolite Repression

Per liter:

20 gm Bacto-tryptone
  5 gm Yeast extract
0.5 gm NaCl
0.186 gm KCl

dissolve in 900 ml Milli-Q water, adjust pH to 7.0 with NaOH, and autoclave.

When the autoclaved solution has cooled to below 60 °C, add 10 ml of filter sterilized 2 M glucose and 10 ml of 1 M magnesium sulfate. Distribute 10 ml to several tubes and store at -80 °C.

Recipe contributed by John Brandis, Ph.D.
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this page last updated Fri, February 12, 2010 14:07